The Effect of a High Dose of Methotrexate on Type II Collagen induced Arthritis in Rats / 대한정형외과학회잡지

PURPOSE: Rat arthritis was induced by bovine type II collagen. To present the remedial effects on type II collagen arthritis and clinical efficacy, a high dose of methotrexate, of a level similar to that used in cancer patients was given to rats. MATERIALS AND METHODS: Healthy male Brown Norway rats were used, of mean weight; 300 g. There were 7 subgroups: a control group, given collagen only, an orally methotrexate treated (2.5mg/week), high methotrexate dose groups; 10 mg/kg, 30 mg/kg, 50 mg/kg, 100 mg/kg and a low dose methotrexate group (0.375 mg/kg/week, 4 times) were included in the present study. Type II collagen was injected for arthritis induction and a single intraperitoneal injection of methotrexate was administered after 15 days. Orally treated group was administered 4 times (2.5 mg/week). Histopathologic findings were evaluated. RESULTS: 50 mg/kg methotrexate was effectively reduced the arthritis index and significantly decreased inflammatory cell number between 2nd and 6 weeks. CONCLUSION: A high dose of methotrexate may be clinically effective in the short period treatment of rheumatoid arthritis patients.

Recycling Autograft with Extracorporeal Irradiation for Reconstruction in Malignent Bone and Soft Tissue Tumors / 대한정형외과학회잡지

PURPOSE: This report is comprehensive results of our clinical series in which we report theoretical advantages and indication of recycling autograft following extracorporeal irradiation in malignant bone and soft tissue tumors. MATERIALS AND METHODS: We have treated in 24 patients by en bloc resection, extracorporeal irradiation and reimplantation of irradiated bone from june 1994 to june 1999. All patients were evaluated radiologiccal union, functional results and complication. RESULTS: The radiologiccal union of junctional sites had appeared at 9.1 months postoperatively. The mean functional results was 73%, we used the criteria of Enneking et al.. There are 10 cases (47.6%) of complication, witch were nonunion, epiphyseal collapse, joint instability, infection, local recurrence and metal failure. CONCLUSION: The recycling autograft following extracorporeal irradiation is a useful method on minimal bony destruction, preserving joint function as osteoarticular autograft, using bone with soft tissue (ligament and tendon) attachment in periarticular tumor and biological spacer in immature skeletal age.